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KMID : 0370019900040000088
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Chung-Ang Journal of Pharmacal Sciences
1990 Volume.4 No. 0 p.88 ~ p.97
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Fluorophotometric Determination of Enoxacin by Modification into Its Dansyl Derivative
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Abstract
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A sensitive and specific method is described for the determination of a new secondary aromatic amine chemotherapeutic drug, Enoxacin. The highly fluorescent Dns-derivative of Enoxacin has been obtained by reacting ethanol solution of Enoxacin with Dns-Cl in acetone and sodium bicarbonate aqueous solution. The Dns-derivative is extracted with methylene chloride and its concentration is measured using a spectrofluorimeter in methanol solution(excitation wavelength : 415nm, emission wavelength : 535nm). The amount of Dns-Cl and 0.1M-NaHCO_3, reaction temperature, reaction time, stability of Dns-derivative and extraction solution were investigated to determine the quantitative potential of the method. Limit of detection is 0.1㎍ / ml with linear response up to 8㎍ / ml and 40㎍ / ml(as Enoxacin in methanol). The fluorescent labelled method is very stable and applicable to the determination of Enoxacin contained in pharmaceutical preparation.
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